The proposed Cycle Superhighway route, running via Birdcage Walk in St James's Park, will not go ahead due to the Royal Parks rejecting the plans.
Royal Parks chief executive Linda Lennon said in the Evening Standard that the Superhighway design would "significantly affect day-to-day park operations".
"A suitable method of separation (of cyclists from vehicles) has not been presented to the Royal Parks to enable this proposal to work satisfactorily.”
As a result, cyclists will now likely be forced to detour to an unprotected route via Horse Guards Road, crossing two risky junctions at The Mall.
Transport for London has previously had to shelve a plan to take the superhighway in front of tourist hotspot Buckingham Palace.
Despite offering "support (for) the overall ambition", Lennon added: “The Royal Parks reserves the right to re-site, reconfigure or remove these routes and infrastructure in future if the impact of the cycle routes lead to increased conflict, undermines safety or has a detrimental impact to the intrinsic qualities of other areas of the park.”
The East to West Cycle Superhighway is expected to be complete by May 2016.
Read more on the Standard here.