To apply for the new scheme, companies have to draw up work-place travel plans, which lay out how they will encourage and help their staff to use sustainable transport like cycling, walking or public transport.
Many companies, including GlaxoSmithKline (GSK) which has focused on cycling, have already seen benefits from providing bike parking and showers, lockers and drying facilities.
Peter Handcock, vice-president, World Wide Real Estate, GSK, saids: "Although we have spent a reasonable amount of money on our cycle measures it is not always the expensive ones that are appreciated the most. A number of the elements cost very little, but the key thing has been using the enthusiasm and commitment of the cyclists themselves to develop improved facilities which attract new people to cycling.
"Getting more people cycling means that we use our buildings more efficiently and adds to creating a more happy and healthy work force. It is not so much a case of 'do-gooding' as of good business."
Ben Plowden, TfL's managing director of group communications, said: "It is estimated that each [car] parking space costs up to £2000 a year to maintain, but five bicycles will fit into a parking space. So, encouraging just five members of staff to travel to work by bicycle could save a company as much as £8000.
"Cycling is seeing an exciting renaissance in the capital, but a lack of cycle parking and changing facilities in the work place are regularly cited as barriers to employees cycling. We want to help businesses remove those barriers and to encourage firms to provide more transport solutions for their staff."
Businesses interested in work place travel plans and the associated offers should contact TfL customer services on tel: 020 7027 6006.